METHODS OF PREPARING ORGANS FOR CRYOPRESERVATION A

专利名称：METHODS OF PREPARING ORGANS FOR

CRYOPRESERVATION AND SUBSEQUENTTRANSPLANTATION

发明人：FAHY, Gregory, M.,KHIRABADI,

Bijan,OKOUCHI, Yasumitsu,MACIAG, Thomas

申请号：EP95928376.0申请日：19950811公开号：EP0777413A1公开日：19970611

摘要：The invention relates to the field of organ and tissue perfusion. More

particularly, the present invention relates to a method for preparing organs, such as thekidney and liver, for cryopreservation through the introduction of vitrifiable

concentrations of cryoprotectant into them. To prepare the organ for cryopreservation,the donor human or animal, is treated in the usual manner and may also be treated withiloprost, or other vasodilators, and/or transforming growth factor &bgr;1. Alternatively,or additionally, the organ which is to be cryopreserved can be administered iloprost, orother vasodilators, and/or transforming growth factor &bgr;1 directly into its artery. Theinvention also relates to preparing organs for transplantation by a method for theremoval of the cryoprotectant therefrom using low (such as raffinose, sucrose, mannitol,etc.), medium (such as agents with intermediate molecular weights of around 600-2,000)and high (such as hydroxyethyl starch) molecular weight agents osmotic buffering agents.The invention is also directed to new post-transplantation treatments such as the use oftransforming growth factor &bgr;1, N-acetylcysteine and aurothioglucose. Further, the

invention relates to a method of vitrification of organs comprising a multi-step method ofintroducing increasing concentrations of cryoprotectant with osmotic equilibration of theorgan and decreases in temperature prior to perfusion with vitrifiable concentrations ofcryoprotectant at about -5° C. to - 35° C.

申请人：THE AMERICAN NATIONAL RED CROSS

地址：17th & D Street, N.W. Washington, DC 20006 US

国籍：US

代理机构：Fuchs, Luderschmidt & Partner

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